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Banik, Ajit Kumar
- Induced Mutation, Development of Multiple Analogue Resistant Strain and Protoplast Fusion for L-Methionine Fermentation by Corynebacterium glutamicum
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Authors
Affiliations
1 Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha, IN
2 Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha
3 Department of Chemical Engineering, Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, West Bengal, IN
1 Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha, IN
2 Post-Graduate Department of Botany, Utkal University, Vani Vihar, Bhubaneswar-751004, Odisha
3 Department of Chemical Engineering, Biochemical Engineering Division, Biotechnology Laboratory, University of Calcutta, West Bengal, IN
Source
Research Journal of Pharmaceutical Dosage Form and Technology, Vol 6, No 1 (2014), Pagination: 1-6Abstract
Corynebacterium glutamicum X1 , a L-glutamic acid producing wild strain which accumulated mild amount of Lmethionine (only 0.6 mg/ml) in the broth ,was subjected to mutational treatments using EMS and UV irradiations to develop a high L-methionine producing mutant. High L-methionine yielding strain Corynebacterium glutamicum X168 was selected in this study which was then subjected to protoplast fusion with a multiple L-methionine analogueresistant strain Corynebacterium glutamicum X124. A high L-methionine yielding, multiple L-methionine analogue resistant mutant Corynebacterium glutamicum X300 was derived in this study which accumulated 9.6 mg /ml Lmethionine in the fermentation broth.Keywords
Corynebacterium glutamicum , L-Glutamic Acid , L-Methionine, Analogue Resistant.- Effect of Osmotic Stress on L-Glutamic Acid Production by an Auxotrophic Mutant Micrococcus glutamicus AB100
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Authors
Affiliations
1 Vidyasagar College, Main Campus, 39 Sankar Ghose Lane, Kolkata 700 006, IN
2 Department of Chemical Engineering, Biochemical Engineering Subdivision, Biotechnology Laboratory, A.P.C Road, Kolkata-700009, IN
1 Vidyasagar College, Main Campus, 39 Sankar Ghose Lane, Kolkata 700 006, IN
2 Department of Chemical Engineering, Biochemical Engineering Subdivision, Biotechnology Laboratory, A.P.C Road, Kolkata-700009, IN